Gallic and ascorbic acids supplementation alleviate cognitive deficits and neuropathological damage exerted by cadmium chloride in Wistar rats.

Cadmium is a highly neurotoxic heavy metal that interferes with DNA repair mechanisms via generation of reactive oxygen species. The potentials of polyphenols and antioxidants as effective protective agents following heavy metal-induced neurotoxicity are emerging. We therefore explored the neuroprotective potentials of gallic and ascorbic acids in CdCl2-induced neurotoxicity. Seventy-two Wistar rats were divided into six groups. Group A received distilled water, B: 3 mg/kg CdCl2, C: 3 mg/kg CdCl2 + 20 mg/kg gallic acid (GA), D: 3 mg/kg CdCl2 + 10 mg/kg ascorbic acid (AA), E: 20 mg/kg GA and F: 10 mg/kg AA orally for 21 days. Depression, anxiety, locomotion, learning and memory were assessed using a battery of tests. Neuronal structure and myelin expression were assessed with histological staining and immunofluorescence. The Morris Water Maze test revealed significant increase in escape latency in CdCl2 group relative to rats concurrently treated with GA or AA. Similarly, time spent in the target quadrant was reduced significantly in CdCl2 group relative to other groups. Concomitant administration of gallic acid led to significant reduction in the durations of immobility and freezing that were elevated in CdCl2 group during forced swim and open field tests respectively. Furthermore, GA and AA restored myelin integrity and neuronal loss observed in the CdCl2 group. We conclude that gallic and ascorbic acids enhance learning and memory, decrease anxiety and depressive-like behavior in CdCl2-induced neurotoxicity with accompanying myelin-protective ability.

Administration of ethanolic extract of Erythrophleum ivorense (A Chev.) stem bark to male Wistar rats alters brain areas involved in motor coordination, behavior, and memory.

ETHNOPHARMACOLOGICAL RELEVANCE: Erythrophleum ivorense (A Chev.) is a common plant in the tropics. Its use as ordeal poison in folklore medicine is controversial. The incoordination and behavioral changes following consumption are often associated with guilt. This study is aimed at dispelling or upholding this belief by investigating the actions of E. ivorense on the brain and behavior using rat model. MATERIALS AND METHODS: Sixty male Wistar rats were equally divided into five groups; control group received distilled water, test groups were administered 10, 20, 30 and 40 mg/kg ethanolic extract of E. ivorense in a daily oral dose for 28 days. Cognition (Morris water maze) depression (forced swim test), motor function (hanging wire and inverted wire mesh grid grip tests) and exploratory assessments were done. Brains were stained with H&E, Cresyl violet and immunohistochemistry was done using GFAP, anticalbindin-D28k, Iba-1 and MBP antibodies. RESULTS: At all doses, E. ivorense significantly (P ≤ 0.05) increased escape latency in the Morris water maze compared to control. Forced swim test showed a dose-related increase in duration of immobility, significant reduction in hanging latency in hanging wire and wire mesh grid grip test was also observed. Depletion of Purkinje cells of the cerebellum and hippocampal neurons was observed with H&E and cresyl violet. Immuno-staining revealed astrocytic activation in the cerebellum, loss of dendritic spines, cortical microglial activation and demyelination in the cerebellum and dentate gyrus of the hippocampus. CONCLUSION: The ethanolic extract of E. ivorense stem bark caused a dose-dependent deficit in learning, memory and motor coordination with evidences of depression in rats. It is concluded that the plant is neurotoxic and induce several neurobehavioral changes.

Polyphenol-rich fraction of Alchornea cordifolia leaf ameliorates arsenite-induced infertility in male rats.

Alchornea cordifolia is used traditionally for the treatment of infertility and venereal diseases. Thirty rats were randomly divided into five groups of six rats each: Group 1 (distilled water), group 2 (7 mg/kg sodium arsenite), group 3 (7 mg/kg sodium arsenite and 100 µg/kg polyphenol-rich fraction 1 of A. cordifolia), group 4 (7 mg/kg sodium arsenite + 100 µg/kg polyphenol-rich fraction 2) and group 5 (7 mg/kg sodium arsenite + α-tocopherol). The experiment lasted 30 consecutive days. Biochemical markers of oxidative stress and antioxidants, male reproductive hormones, spermatozoa function tests, histopathology, immunoreactivity of androgen receptor binding protein (ARBP) and anti-apoptotic B-cell lymphoma-2 protein expressions were estimated. Data were analysed using descriptive statistics and ANOVA at p < .05. Treatment with AF1 significantly decreased markers of oxidative stress, but increased the systemic antioxidants, testosterone, FSH, spermatozoa count and motility. Histopathological lesions of necrosis and germinal epithelial sloughing observed in sodium arsenite group were absent in sodium arsenite + 100 µg/kg polyphenol-rich fraction 1 group. Expressions of androgen receptor binding protein and anti-apoptotic B-cell lymphoma-2 were highest in sodium arsenite + 100 µg/kg polyphenol-rich fraction 1 group. In conclusion, the polyphenol-rich fraction of A. cordifolia is protective against sodium arsenite-induced infertility in male rats through the inhibition of oxidative and apoptotic mechanisms.

Adverse reproductive effects of ethanolic root extract of Waltheria indica in male Wistar rats.

Background Numerous uses of Waltheria indica plant such as antitrypanosomal, antibacterial and antimalarial effects have been reported. It has however been reported that most plants with antibacterial and antiprotozoal effects have adverse effect on male reproduction. Hence, we evaluated the effect of Waltheria indica root on male reproductive parameters. Methods Twenty adult male Wistar rats were randomly divided into four groups (n=5); A-D. Group A served as control group while groups B, C and D were administered with 200, 400 and 800 mg/Kg body weight of crude ethanolic extract of Waltheria indica root. After 28 days of administration, the rats were sacrificed and sperm parameters, sperm morphology, serum reproductive hormones and lipids were determined. Results There was a significant reduction in sperm count and motility as well as significant increase in percentage abnormal sperm cell (p<0.001) at the 400 and 800 mg/kg BW. The serum levels of testosterone was also significantly reduced while total cholesterol increased significantly (p<0.05) at the highest dose. Conclusion Waltheria indica root has adverse effect on male reproduction through reduction in sperm parameters and male reproductive hormones.

Βeta-sitosterol enhances motor coordination, attenuates memory loss and demyelination in a vanadium-induced model of experimental neurotoxicity.

Environmental discharge of vanadium causes cognitive and behavioral impairments in humans and animals via production of reactive oxygen species leading to lipid peroxidation and alteration in antioxidant defence system. The current study was carried out to investigate the cognitive-enhancing ability of ß-sitosterol in vanadium-induced neurotoxicity. Forty eight mice were randomly assigned into 4 groups (A-D) with the following treatments: group A; distilled water, B; α-tocopherol + sodium metavanadate (NaO3V), C; ß-sitosterol + NaO3V and D; only NaO3V. NaO3V was administered intraperitoneally while other treatments were administered through gavage for 7 consecutive days. Neurobehavioral parameters measuring cognition, locomotion, anxiety and grip strength were evaluated at day 8. Following sacrifice, brain levels of catalase, superoxide dismutase, glutathione, malonaldehyde (MDA) and hydrogen peroxide (H2O2) were measured. Immunohistochemical expression of Myelin Basic Protein (MBP) in the brain was also investigated. The results showed that deficits in spatial learning, locomotor efficiency, and motor coordination, induced by acute vanadium neurotoxicity were mitigated by beta-sitosterol. Significantly (α ≤ 0.05) decreased in vivo antioxidant enzyme activities, increased brain levels of MDA and H2O2, structural damage to myelin sheaths and decreased expression of MBP were also observed in the NaO3V group (D), however, co-administration of ß-sitosterol reduced these pathologic features. It is concluded that ß-sitosterol alleviates vanadium-induced neurotoxicity by enhancing cognition and improving motor co-ordination via its antioxidant and myelo-protective activities.

Sodium metavanadate induced cognitive decline, behavioral impairments, oxidative stress and down regulation of myelin basic protein in mice hippocampus: Ameliorative roles of ß-spinasterol, and stigmasterol.

INTRODUCTION: Exposures to toxic levels of vanadium and soluble vanadium compounds cause behavioral impairments and neurodegeneration via free radical production. Consequently, natural antioxidant sources have been explored for effective and cheap remedy following toxicity. Grewia carpinifolia has been shown to improve behavioral impairments in vanadium-induced neurotoxicity, however, the active compounds implicated remains unknown. Therefore, this study was conducted to investigate ameliorative effects of bioactive compounds from G. carpinifolia on memory and behavioral impairments in vanadium-induced neurotoxicity. METHODS: Sixty BALB/c mice were equally divided into five groups (A-E). A (control); administered distilled water, B (standard); administered α-tocopherol (500 mg/kg) every 72 hr orally with daily dose of sodium metavanadate (3 mg/kg) intraperitoneally, test groups C, and D; received single oral dose of 100 µg ß-spinasterol or stigmasterol (bioactive compounds from G. carpinifolia), respectively, along with sodium metavanadate and the model group E, received sodium metavanadate only for seven consecutive days. Memory, locomotion and muscular strength were accessed using Morris water maze, Open field and hanging wire tests. In vivo antioxidant and neuroprotective activities were evaluated by measuring catalase, superoxide dismutase, MDA, H2 O2 , and myelin basic protein (MBP) expression in the hippocampus. RESULTS: In Morris water maze, stigmasterol significantly (p ≤ 0.05) decreased escape latency and increased swimming time in target quadrant (28.01 ± 0.02; 98.24 ± 17.38 s), respectively, better than α-tocopherol (52.43 ± 13.25; 80.32 ± 15.21) and ß-spinasterol (42.09 ± 14.27; 70.91 ± 19.24) in sodium metavanadate-induced memory loss (112.31 ± 9.35; 42.35 ± 11.05). ß-Spinasterol and stigmasterol significantly increased exploration and latency in open field and hanging wire tests respectively. Stigmasterol also increased activities of antioxidant enzymes, decreased oxidative stress markers and lipid peroxidation in mice hippocampal homogenates, and increased MBP expression. CONCLUSIONS: The findings of this study indicate a potential for stigmasterol, a bioactive compound from G. carpinifolia in improving cognitive decline, motor coordination, and ameliorating oxidative stress in vanadium-induced neurotoxicity.

Modulatory effects of melatonin and vitamin  C on oxidative stress-mediated haemolytic anaemia and associated cardiovascular dysfunctions in rats.

Background Phenylhydrazine (PHE) in experimental animal models has been widely reported to cause haemolytic anaemia, via the induction of oxidative stress and thus causing deleterious cardiovascular complications. Hence, this study was designed to evaluate the possible modulatory role of melatonin (MLT) or vitamin C when co-administered with PHE. Methods Anaemia was established with PHE administration. MLT or vitamin C was co-administered with PHE. Haematological parameters, markers of oxidative stress, enzymic and non-enzymic antioxidants, blood pressure and electrocardiograms were assessed. Results PHE administration led to a significant (p<0.05) increase in malondialdehyde (MDA), and hydrogen peroxide (H2O2) generated in cardiac, renal and red blood cell (RBC) lysates. PHE also significantly reduced the activity of glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) contents, respectively. The RBC counts, haemoglobin (Hb) concentration and packed cell volume (PCV) were also significantly reduced following the administration of PHE. Furthermore, the systolic blood pressure (SBP), diastolic blood pressure (DBP) and mean arterial blood pressure (MABP) increased significantly in rats administered PHE alone. Similarly, PHE administration led to a significant drop in heart rate but prolonged QRS, QT and QTc interval. Pathology of the heart and kidney was also observed in PHE treated group. However, treatment with MLT and vitamin C improved enzymic and non-enzymic antioxidant system together with the restoration of SBP, DBP and MABP to near normal. The architectural anarchy observed in the heart and kidney of PHE administered rats was reversed to some extent. Conclusions Hence, MLT and vitamin C could be employed as therapeutic targets in various cardiovascular diseases and its complications.

Behavioural studies on the ethanol leaf extract of Grewia carpinifolia in Wistar rats.

BACKGROUND: Grewia carpinifolia is a plant commonly used in the tropics to manage various central nervous system (CNS) disorders. However, despite its widespread use no scientific work has been reported to validate these claims. OBJECTIVES: To evaluate the activity of G. carpinifolia as it affects behaviour using animal model. METHODS: Twenty five adult Wistar rats were randomly divided into five groups (A-E). Group A served as control (given only distilled water), Groups B, C, D and E were administered with single oral dose of ethanol extract of G. carpinifolia leaf at 100, 200, 400 and 800 mg/kg body weight respectively for twenty eight days consecutively. Subsequently, open field test, negative geotaxis and hanging wire test were performed. Body and brain weights were measured and histological examination of the brain was also performed. RESULTS: At the tested doses, the extract significantly increased the time spent on the hanging wire and decreased locomotor activity at 800 mg/kg. No significant difference was observed in body and brain weights of extract treated groups when compared with the control. No visible histological lesion was also observed. CONCLUSION: The plant extract may improve muscular strength at tested doses and possess CNS depressant activity at 800 mg/kg.

Phytochemical screening and toxicity studies on the methanol extract of the seeds of moringa oleifera.

The seeds of Moringa oleifera were collected, air-dried, pulverized, and subjected to cold extraction with methanol. The methanol extract was screened phytochemically for its chemical components and used for acute and sub-acute toxicity studies in rats. The phytochemical screening revealed the presence of saponins, tannins, terpenes, alkaloids, flavonoids, carbohydrates, and cardiac glycosides but the absence of anthraquinones. Although signs of acute toxicity were observed at a dose of 4,000 mg kg-1 in the acute toxicity test, and mortality was recorded at 5,000 mg kg-1, no adverse effect was observed at concentrations lower than 3,000 mg kg-1. The median lethal dose of the extract in rat was 3,873 mg kg-1. Sub-acute administration of the seed extract caused significant (p<0.05) increase in the levels of alanine and aspartate transferases (ALT and AST), and significant (p<0.05) decrease in weight of experimental rats, at 1,600 mg kg-1. The study concludes that the extract of seeds of M. oleifera is safe both for medicinal and nutritional uses.

Assessment of the effects of different concentrations of ethylenediaminetetraacetic acid (EDTA) on erythrocytic indices of Nigerian White Fulani cattle and West African Dwarf goat.

The aim of the study was to evaluate the effects of varying concentrations of ethylenediaminetetraacetic acid (EDTA) on blood samples from White Fulani breed of cattle and West African Dwarf goat from Nigeria. Sample sizes of 20 animals were used for both species. Different concentrations of EDTA (2, 4, 8 and 16 mg/ml) were used. The packed cell volume (PCV), red blood cell (RBC) and haemoglobin (Hb) concentration of blood samples collected from White Fulani breed of cattle and West African Dwarf goat into bottles containing 16 mg/ml of EDTA were significantly lower (P < 0.05) than those samples collected from the same animals into bottle containing 2 mg/ml (control). Similarly, the PCV, RBC and Hb values of the West African Dwarf goats in bottles containing 8 mg/ml of EDTA were significantly lower than those of the samples in the control (2 mg/ml). This study has shown that high concentration of EDTA as an anticoagulant can lead to a false erythrocytic index especially the PCV. In collecting blood samples for evaluation of haematological parameters, therefore, the blood volume/anticoagulant ratio must be strictly adhered to prevent error in the evaluated parameters in cattle and goats. Taken together, there is tendency for haemolytic anaemia to occur in blood sampled at higher concentration of anticoagulants in West African Dwarf goat than in White Fulani breed of cattle.